Symbiotic Fungus of Marine Sponge Axinella sp. Producing Antibacterial Agent

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Trianto, A.^1,2 ; Widyaningsih, S.^3 ; Radjasa, O.K.^1,2 ; Pribadi, R.^1

Natural Product Laboratory, Centre of Research and Services (Laboratorium Terpadu), Diponegoro University, Indonesia^1

Department Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Indonesia^2

Magister of Marine Sciences, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Indonesia^3

关键词: Axinella sp;    Bioactive compounds;    Molecular analysis;    multidrug resistace;    Multidrug resistance;    Pathogenic bacterium;    sponge;    Staphylococcus aureus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012005/pdf
DOI  :  10.1088/1755-1315/55/1/012005

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The emerging of multidrug resistance pathogenic bacteria cause the treatment of the diseaseshave become ineffective. There for, invention of a new drug with novel mode of action is an essential for curing the disease caused by an MDR pathogen. Marine fungi is prolific source of bioactive compound that has not been well explored. This study aim to obtain the marine sponges-associated fungus that producing anti-MDR bacteria substaces. We collected the sponge from Riung water, NTT, Indonesia. The fungus was isolated with affixed method, followed with purification with streak method. The overlay and disk diffusion agar methods were applied for bioactivity test for the isolate and the extract, respectively. Molecular analysis was employed for identification of the isolate. The sponge was identified based on morphological and spicular analysis. The ovelay test showed that the isolate KN15-3 active against the MDR Staphylococcus aureus and Eschericia coli. The extract of the cultured KN15-3 was also inhibited the S. aureus and E. coli with inhibition zone 2.95 mm and 4.13 mm, respectively. Based on the molecular analysis, the fungus was identified as Aspergillus sydowii. While the sponge was identified as Axinella sp.