FEBS Letters | |
Phosphorylation of elongation factor‐2 from the lepidopteran insect, spodoptera frugiperda | |
Oldfield, Susan1  Proud, Christopher G.1  | |
[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK | |
关键词: Elongation factor 2; Protein synthesis; Phosphorylation; Spodoptera; (eEF-2); Eukaryotic elongation factor 2; MES; 2-[N-morpholino]ethanesulphonic acid; SDS PAGE; sodium dodecyl sulphate/polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(93)81042-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In mammalian cells, protein synthesis can be regulated at the level of elongation by the phosphorylation of elongation factor 2 (eEF-2) by a highly specific Ca2+/calmodulin-dependent kinase. In this report, we show that eEF-2 from a cell line derived from the insect, Spodoptera frugiperda, is a substrate for mammalian eEF-2 kinase and that phosphorylation is Ca2+-dependent. Furthermore, two-dimensional peptide mapping shows that the kinase phosphorylates the same sites in Spodoptera eEF-2 as those phosphorylated in the rabbit protein. However, we were unable to detect an eEF-2 kinase in Spodoptera cells.
【 授权许可】
Unknown
【 预 览 】
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