Phosphorylation of elongation factor‐2 from the lepidopteran insect, spodoptera frugiperda

FEBS Letters

Oldfield, Susan¹  Proud, Christopher G.¹ 

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK

关键词: Elongation factor 2;    Protein synthesis;    Phosphorylation;    Spodoptera;    (eEF-2);    Eukaryotic elongation factor 2;    MES;    2-[N-morpholino]ethanesulphonic acid;    SDS PAGE;    sodium dodecyl sulphate/polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(93)81042-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In mammalian cells, protein synthesis can be regulated at the level of elongation by the phosphorylation of elongation factor 2 (eEF-2) by a highly specific Ca²⁺/calmodulin-dependent kinase. In this report, we show that eEF-2 from a cell line derived from the insect, Spodoptera frugiperda, is a substrate for mammalian eEF-2 kinase and that phosphorylation is Ca²⁺-dependent. Furthermore, two-dimensional peptide mapping shows that the kinase phosphorylates the same sites in Spodoptera eEF-2 as those phosphorylated in the rabbit protein. However, we were unable to detect an eEF-2 kinase in Spodoptera cells.

【 授权许可】

Unknown