Producing armyworm (spodoptera sp.) Bioinsecticide based on cysteine protease of red ginger (zingiber officinale var. Rubrum)

Quality in Research: International Symposium on Materials, Metallurgy, and Chemical Engineering

材料科学;冶金学;化学工业

Afnan, N.T.^1 ; Nur, D.F.^1 ; Utami, T.S.^1 ; Sahlan, M.^1 ; Wijanarko, A.^1 ; Hermansyah, H.^1,2

Bioprocess Engineering, Engineering Faculty, University of Indonesia, Depok

16424, Indonesia^1

DRPM Universitas Indonesia, University of Indonesia, Depok

16424, Indonesia^2

关键词: Acetylcholinesterase;    Bioinsecticides;    Cysteine protease;    Spodoptera;    Zingiber officinale;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/316/1/012062/pdf
DOI  :  10.1088/1757-899X/316/1/012062

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Armyworm (Spodoptera sp.) is highly polyphagous defoliator on various horticulture and grain plants. Various chemical insecticides have been created to control it. There is a need to create an eco-friendly and specific insecticide which only affect armyworm's nervous system. This research investigates cysteine-protease's enzyme activity of red ginger (Zingiber officinale var. Rubrum) which is called zingibain. Its catalytic site matches with residue site in armyworm's body so it can be used as bioinsecticide raw material which meets the criterias above. Fresh red ginger rhizomes were washed and extracted. The juice was then deposited in low temperature and centrifuged to get rid of its starch content. It was filtrated to remove large contaminants and poured into Potassium Phospate buffer. The liquid was then centrifuged again for 30 minutes before collecting the supernatant. Fresh leaves were then dipped into crude ginger protease extract and fed to fourth instar-armyworms. Leaves dipped into non-diluted extract were barely eaten by armyworm while the 50% and 25% dilution was half eaten and most eaten. The crude red ginger extract was not strong enough to kill them although the research showed its enzymatic activity reaches up to 169 PU. It still needs improvement to be produced as commercial bioinsecticide.