Prostaglandin A2 and Δ12‐prostaglandin J2 induce apoptosis in L1210 cells

FEBS Letters

Sohn, Hae-Won²  Kim, Sang-Ho¹  Lee, Jeong-Hwa²  Kim, Ho-Shik²  Kim, In-Kyung² 

[1] Department of Pathology, Catholic University Medical College, Seoul 137-701, South Korea;Department of Biochemistry, Catholic University Medical College, Seoul 137-701, South Korea

关键词: Δ12-prostaglandin J2;    Antiproliferative activity;    DNA fragmentation;    Apoptosis;    PG;    prostaglandin;    Δ12-PGJ2;    9-deoxy-Δ9;    12-13;    14-dihydro PGD2;    TNF;    tumor necrosis factor;    TGF-β;    transforming growth factor-β;   

DOI  :  10.1016/0014-5793(93)80110-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of L1210 cells with prostaglandin A₂ (PGA₂) or 9-deoxy-Δ^9,12-13,14-dihydro PGD₂ (Δ¹²-PGJ₂) resulted in significant G2/M arrest and subsequent DNA fragmentation at concentrations that are cytotoxic to the cells. On agarose gel electrophoresis, DNA ladder formation was evident 24 h after the addition of Δ¹²-PGJ₂ and remained apparent through 72 h, whereas G2/M accumulation was observed 6 h after the treatment. When the morphology of cells was examined by electron microscopy, L1210 cells incubated with a cytotoxic dose of PGA₂ or Δ¹²-PGJ₂ for 24 h showed the characteristic morphological features of apoptosis such as chromatin condensation, nuclear fragmentation and formation of apoptotic body. Cycloheximide blocked the DNA fragmentation and morphological changes induced by Δ¹²-PGJ₂. Our results suggest that these cyclopentenone PGs caused apoptotic cell death of L1210 cells which is preceded by G2/M accumulation and requires de novo protein synthesis.

【 授权许可】

Unknown