DNA Damage Observed in Unaffected Individuals with Family History of T2DM

14th International Conference on Science, Engineering and Technology

自然科学;工业技术

Ramesh, Nikhila^1 ; Abilash, V.G.^1

Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu

632014, India^1

关键词: Alkaline comet assay;    DNA fragmentation;    Environmental factors;    Genetic alterations;    Genomic stability;    Human leukocytes;    Reactive oxygen species;    Type 2 diabetes mellitus;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/263/2/022037/pdf
DOI  :  10.1088/1757-899X/263/2/022037

来源: IOP

【 摘 要 】

Diabetes has been documented to cause high levels of DNA fragmentation in some cases. As diabetes is inheritable and influenced by both genetic and environmental factors, an investigation into the genomic stability of individuals who are strongly at risk of inheriting diabetes was conducted by inducing oxidative stress, as DNA damage in unaffected individuals could be a sign of onset of the disease or the presence of genetic alterations that reduce cellular defences against reactive oxygen species. In this study, alkaline comet assay was performed on isolated human leukocytes to determine whether individuals with a family history of Type 2 Diabetes Mellitus (T2DM) are more prone to DNA damage under oxidative stress. Visual scoring of comets showed that these individuals have higher degree of DNA damage compared to a control individual with no family history of Type 2 Diabetes Mellitus. Further studies with large sample could determine the presence of disabled cellular defences against oxidative stress in unaffected individuals and intervention with antioxidants could prevent or manage Type 2 Diabetes Mellitus and its complications.