Properties of engineered antifreeze peptides

FEBS Letters

Hague, Charles M.¹  Warren, Gareth J.¹  Mueller, Gunhild M.¹  Corotto, Loren V.¹ 

[1] DNA Plant Technology Corp., 6701 San Pablo Avenue, Oakland, CA 94608, USA

关键词: Antifreeze peptide;    Pseudopleuronectes americanus;    HPLC;    reverse phase;    Protein engineering;    Ice;    recrystallization;   

DOI  :  10.1016/0014-5793(93)80090-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Eight antifreeze-like peptides were produced by cleavage from engineered chimeric proteins. One was homologous to an antifreeze peptide of the winter flounder; the others differed in length and/or sequence. The homologous peptide and all those of equal or greater length were able to inhibit recrystallization. The longer peptides were so hydrophobic that their identification required modification of the usual protocols for high pressure liquid chromatography. Their elution positions were correlated to their hydrophobicities and their lengths. Additional naturally occurring antifreezes may be identifiable with this knowledge.

【 授权许可】

Unknown