Disulfide bridges in human complement component C3b

FEBS Letters

Sottrup-Jensen, Lars¹  Dolmer, Klavs¹ 

[1] Department of Molecular Biology, University of Aarhus, Bldg. 130, Århus C, Denmark

关键词: Plasma protein;    Complement C3;    Disulfide bridge;    Sequence homology;    α2-Macroglobulin superfamily kw|abrC3(MA);    C3 treated with methylamine and iodoacetamide;    α2M;    α2-macroglobulin;    HPLC;    high-performance liquid chromatography;    RP;    reverse phase;    SCX;    strong cation exchange;    TFA;    trifluoroacetic acid;    PTH;    phenylthiohydantoin.;   

DOI  :  10.1016/0014-5793(93)81139-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The disulfide bridges of human complement component C3b, derived from C3 by removal of the 77-residue C3a, have been determined. The 10 bridges are Cys⁵³⁷-Cys⁷⁹⁴, Cys⁶⁰⁵-Cys⁶⁴⁰, Cys⁸⁵¹-Cys¹⁴⁹¹, Cys¹⁰⁷⁹-Cys¹¹⁶⁹, Cys¹³³⁶-Cys¹⁴⁶⁷, Cys¹³⁶⁷-Cys¹⁴³⁶, Cys¹⁴⁴⁸-Cys¹⁴⁸⁹, Cys¹⁴⁹⁶-Cys¹⁵⁶⁸, Cys¹⁵¹⁵-Cys¹⁶³⁹, and Cys¹⁶¹⁵-Cys¹⁶²⁴. Including the 3 bridges in C3a (Cys⁶⁷⁰-Cys⁶⁹⁸, Cys⁶⁷²-Cys⁷⁰⁵, and Cys⁶⁸⁵-Cys⁷⁰⁶) previously determined by high-resolution X-ray crystallography [Hoppe-Seyler's Z. Physiol. Chem. 361 (1980) 1389-1399] all disulfide bridges of C3 are localized. C3 and the strongly related C4 and C5 are members of the α₂-macroglobulin superfamily. The predicted bridge patterns of C4 and C5 are discussed and compared with that of α₂-macroglobulin.

【 授权许可】

Unknown