Brefeldin A arrests the intracellular transport of a precursor of complement C3 before its conversion site in rat hepatocytes

FEBS Letters

Takatsuki, Akira³  Misumi, Yoshio¹  Oda, Kimimitsu¹  Hirose, Shinichi¹  Ikehara, Yukio¹  Takami, Noboru² 

[1] Department of Biochemistry, Fukuoka University School of Medicine, Jonan-ku, Fukuoka 814-01 Japan;Radioisotope Laboratory, Fukuoka University School of Medicine, Jonan-ku, Fukuoka 814-01 Japan;Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Brefeldin A;    Secretion-blocking agent;    Complement C3;    Complement precursor accumulation;    (Rat hepatocyte);    C3;    the third component of complement;    MEM;    minimum essential medium;   

DOI  :  10.1016/0014-5793(87)80028-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of brefeldin A on intracellular transport and posttranslational modification of complement C3 (C3) were studied in primary culture of rat hepatocytes. In the control culture C3 was synthesized as a pre-cursor (pro-C3), which was processed to the mature form with α- and β-subunits before its discharge into the medium. In the presence of brefeldin A the secretion of C3 was strongly blocked, resulting in accumulation of pro-C3. However, after a prolonged interval the mature form of C3 was finally secreted. The results indicate that brefeldin A impedes translocation of pro-C3 to the Golgi complex where pro-C3 is converted to the mature form, but not its proteolytic processing, in contrast to the effects of monensin and weakly basic amines.

【 授权许可】

Unknown