A new distinct group of 2 S albumins from rapeseed

FEBS Letters

Villalba, Mayte¹  Rodríguez, Rosalía¹  Lopez-Otin, Carlos²  Monsalve, Rafael I.¹ 

[1]Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain

[2]Departamento de Biologia Functional, Facultad de Medicina, Universidad de Oviedo, Oviedo, Spain

关键词: 2 S albumin;    Rapeseed;    Primary structure;    RP;    reverse phase;    HPLC;    high-performance liquid chromatography;    TFA;    trifluoroacetic acid;    2-ME;    2-mercaptoethanol;    PTH;    phenylthiohydantoin;    LMW;    low molecular weight;   

DOI  :  10.1016/0014-5793(91)81419-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two napins (nIa and nIb), isolated from Brassica napus (rapeseed) seeds, have been sequenced. The two proteins show the common structural pattern of the 2 S albumins, since they are composed of two disulfide-linked chains of different size, yet they exhibit an atypical low molecular weight (12.5 kDa vs. 14.5 kDa of the major napins). High sequence similarity has been found between these 2 proteins, but only 54% similarity can be estimated from their comparison with the 14.5 kDa major napins. Thus, nIa and nIb are considered representatives of a new distinct group of rapeseed napins since all the previously known napins exhibit 95% sequence similarity. Unexpectedly, the similarity increases when compared with the 2 S proteins from other species.

【 授权许可】

Unknown