Gelation of liposome interior A novel method for drug encapsulation

FEBS Letters

Stuart, M.C.A.⁴  Lasic, D.D.¹  McIntosh, T.J.²  Frederik, P.M.⁴  Barenholz, Y.³ 

[1] Liposome Technology, Inc., Menlo Park, CA, USA;Department of Cell Biology, Duke University, Durham, NC, USA;Department of Biochemistry, Hebrew University, Jerusalem, Israel;University of Limburg, Department of Pathology, Electron Microscopy, Maastricht, The Netherlands

关键词: Liposome;    Drug loading;    (NH4)2SO4;    gradient;    Doxorubicin;    Gel;   

DOI  :  10.1016/0014-5793(92)80947-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Liposomes can be loaded with weak acids and bases, which exist in solutions in equilibrium with membrane permeable uncharged form, using various gradients across their membranes. Because in some cases the estimated drug concentration in the loaded liposomes exceeds their aqueous solubility we investigated the physical state of the liposome encapsulated anticancer drug Doxorubicin. X-Ray diffraction, electron microscopy and test tube solubility experiments have shown that upon encapsulation the drug molecules form a gel-like phase

【 授权许可】

Unknown