Carbohydrates in mammalian tryptophanyl‐tRNA synthetase

FEBS Letters

Kisselev, Lev L.²  Nikitushkina, Tatyana V.²  Egorov, Tsezi A.²  Musoljamov, Aleksander Ch.²  Kovaleva, Galina K.²  Zheltova, Anna O.¹ 

[1] Zelinsky Institute of Organic Chemistry, The Russian Academy of Sciences, Moscow 117334, Russia;Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, Moscow 117984, Russia

关键词: Tryptophanyl-tRNA synthetase;    Beef;    Sugar;    Glycoprotein;    Mammal;    Aminoacyl-tRNA synthetase;    AmS;    ammonium sulfate;    DTT;    dithiotreitol;    2-ME;    2-mercaploethanol;    WRS;    bovine tryptophanyl-tRNA synthetase;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(92)80802-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5–3.0% (w/w) of the enzyme composed of two subunits (60 kDa × 2). The same set of sugars was detected in clastase and CNBr-generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl-tRNA synthetase, in addition to being a metallo- and phosphoprotein, is also a glycoprotein.

【 授权许可】

Unknown