FEBS Letters | |
Ap3A and Ap4A are primers for oligoadenylate synthesis catalyzed by interferon‐inducible 2–5A synthetase | |
Justesen, Just1  Kisselev, Lev2  Turpaev, Kyril1  Hartmann, Rune1  | |
[1] Department of Molecular and Structural Biology, Aarhus University, Aarhus, Denmark;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia | |
关键词: Tryptophanyl-tRNA synthetase; 2-5A synthetase; Ap3A; Ap4A; aaRS; aminoacyl-tRNA synthetase; BSA; bovine serum albumin; FPLC; fast protein liquid chromatography; IFN; interferon; PEI; polyethylene imine; TLC; thin layer chromatography; WRS; tryptophanyl-tRNA synthetase; | |
DOI : 10.1016/S0014-5793(97)00365-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The biological role of Ap3A synthesized in cells by tryptophanyl-tRNA synthetase (WRS) is unknown. Previously we have demonstrated that the cellular level of Ap3A significantly increases after interferon treatment. Here we show that the human 46 kDa 2-5A synthetase efficiently utilizes Ap3A as a primer for oligoadenylate synthesis. The K m for Ap3A is several-fold lower than for Ap4A and 100-fold lower than for ATP. This implies that Ap3A might be a natural primer for the 2′-adenylation reaction catalysed by 2-5A synthetase. Since WRS and 2-5A synthetase are both interferon-inducible proteins, a new link between two interferon-dependent enzymes is established.
【 授权许可】
Unknown
【 预 览 】
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RO201912020304310ZK.pdf | 509KB | download |