Ap3A and Ap4A are primers for oligoadenylate synthesis catalyzed by interferon‐inducible 2–5A synthetase

FEBS Letters

Justesen, Just¹  Kisselev, Lev²  Turpaev, Kyril¹  Hartmann, Rune¹ 

[1] Department of Molecular and Structural Biology, Aarhus University, Aarhus, Denmark;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

关键词: Tryptophanyl-tRNA synthetase;    2-5A synthetase;    Ap3A;    Ap4A;    aaRS;    aminoacyl-tRNA synthetase;    BSA;    bovine serum albumin;    FPLC;    fast protein liquid chromatography;    IFN;    interferon;    PEI;    polyethylene imine;    TLC;    thin layer chromatography;    WRS;    tryptophanyl-tRNA synthetase;   

DOI  :  10.1016/S0014-5793(97)00365-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biological role of Ap₃A synthesized in cells by tryptophanyl-tRNA synthetase (WRS) is unknown. Previously we have demonstrated that the cellular level of Ap₃A significantly increases after interferon treatment. Here we show that the human 46 kDa 2-5A synthetase efficiently utilizes Ap₃A as a primer for oligoadenylate synthesis. The K _m for Ap₃A is several-fold lower than for Ap₄A and 100-fold lower than for ATP. This implies that Ap₃A might be a natural primer for the 2′-adenylation reaction catalysed by 2-5A synthetase. Since WRS and 2-5A synthetase are both interferon-inducible proteins, a new link between two interferon-dependent enzymes is established.

【 授权许可】

Unknown