Carbon‐by‐carbon discrimination of 13C incorporation into liver fatty acids

FEBS Letters

Cunnane, S.C.¹ 

[1] Department of Nutritional Sciences, Faculty of Medicine University of Toronto, Toronto, Canada

关键词: Fatty acid;    [13C]Acetate;    13C NMR;    Liver;   

DOI  :  10.1016/0014-5793(92)81016-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Quantitative carbon-by-carbon analysis would be useful in determining the origin and fate of carbons involved in fatty acid metabolism. Incorporation of ¹³C from 2-[¹³C]acetate into specific carbons of liver fatty acids was lowest at the n—2 carbon of saturates and monoenes but was 47% greater at acyl Cl than at C2, suggesting substantial redistribution of the ¹³C from C2 to Cl of acetyl CoA or malonyl CoA prior to ¹³C incorporation into fatty acids during de novo synthesis or during elongation. Thus, ¹³C derived from exogenous acetate can be quantitatively measured and is differentially incorporated into individual carbons depending on position in the fatty acid molecule.

【 授权许可】

Unknown