Study on Extraction, Isolation and Structure Identification of the Chemical Constituents of Hippeastrum vittatum

1st International Global on Renewable Energy and Development

Yu-Bin, J.I.^1,2 ; Qin-Bing, Xue^1,2 ; Guo-Song, Xin^1,2 ; Xin, Li^1,2

Life Sci. and Environ. Science Research Center of Harbin University of Commerce

Heilongjiang Harbin, 150076, China^1

Natl. Ministry of Education Anti-Tumor Natural Drug Engineering Research Center

Heilongjiang Harbin, 150076, China^2

关键词: 13C NMR;    Cellular proliferations;    Chemical constituents;    MTT assays;    N-butanol;    Physical and chemical properties;    Spectroscopic data;    Structure identification;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/100/1/012047/pdf
DOI  :  10.1088/1755-1315/100/1/012047

来源: IOP

【 摘 要 】

The MTT assay results show that the four parts extraction of amaryllis leaves significantly and dose-dependently inhabit HepG2 cellular proliferation. After Separate and purify the compounds in n-butanol section by multiplex chromatography, two compounds were acquired. According to the date of 1H-NMR, 13C-NMR, MS and other spectroscopic data along with the physical and chemical properties, two compounds structure are identified, namely 2S-7,3'-dihydroxy-4'-methoxy-flavan and (2S)-7-hydroxy-3",4'-dimethoxy-flavan. This two compounds is isolated from Hippeastrum.