Chondroitin-Sulfate-A-Coated Magnetite Nanoparticles: Synthesis, Characterization and Testing to Predict Their Colloidal Behavior in Biological Milieu

International Journal of Molecular Sciences

Etelka Tombácz¹  IldikóY. Tóth²  István Zupkó³  Erzsébet Illés⁴  Márta Szekeres⁴  Rodica Turcu⁵ 

[1] D for Isotopic and Molecular Technology, Donat Street 67-103, 400293 Cluj-Napoca, Romania;Department of Applied and Environmental Chemistry, University of Szeged, Rerrich Béla tér 1, H-6720 Szeged, Hungary;Department of Pharmacodynamics and Biopharmacy, University of Szeged, Eötvös u. 1, H-6720 Szeged, Hungary;Department of Physical Chemistry and Materials Science, University of Szeged, Rerrich Béla tér 1, H-6720 Szeged, Hungary;;National Institute R&

关键词: magnetite;    chondroitin-sulfate;    adsorption;    core-shell nanoparticles;    surface complexation;    surface spectroscopy;    chemical stability;    coagulation kinetics;    colloidal stability;    MTT assays;   

DOI  :  10.3390/ijms20174096

来源: DOAJ

【 摘 要 】

Biopolymer coated magnetite nanoparticles (MNPs) are suitable to fabricate biocompatible magnetic fluid (MF). Their comprehensive characterization, however, is a necessary step to assess whether bioapplications are feasible before expensive in vitro and in vivo tests. The MNPs were prepared by co-precipitation, and after careful purification, they were coated by chondroitin-sulfate-A (CSA). CSA exhibits high affinity adsorption to MNPs (H-type isotherm). We could only make stable MF of CSA coated MNPs (CSA@MNPs) under accurate conditions. The CSA@MNP was characterized by TEM (size ~10 nm) and VSM (saturation magnetization ~57 emu/g). Inner-sphere metal−carboxylate complex formation between CSA and MNP was proved by FTIR-ATR and XPS. Electrophoresis and DLS measurements show that the CSA@MNPs at CSA-loading > 0.2 mmol/g were stable at pH > 4. The salt tolerance of the product improved up to ~0.5 M NaCl at pH~6.3. Under favorable redox conditions, no iron leaching from the magnetic core was detected by ICP measurements. Thus, the characterization predicts both chemical and colloidal stability of CSA@MNPs in biological milieu regarding its pH and salt concentration. MTT assays showed no significant impact of CSA@MNP on the proliferation of A431 cells. According to these facts, the CSA@MNPs have a great potential in biocompatible MF preparation for medical applications.

【 授权许可】

Unknown