A gene family homologous to the S‐phase specific gene in higher plants is essential for cell proliferation in Saccharomyces cerevisiae

FEBS Letters

Yasui, Akira¹  Komamine, Atsushi²  Ito, Masaki² 

[1] Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai 980, Japan;Biological Institute, Faculty of Science, Tohoku University, Sendai 980, Japan

关键词: Cell cycle;    Gene sequence;    cyc07;    Catharanthus roseus;    Saccharomyces cerevisiae;    ORF;    open reading frame;    PCR;    polymerase chain reaction;    ts;    temperature sensitive;    YEPD;    yeast extract-peptone-dextrose;   

DOI  :  10.1016/0014-5793(92)80203-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously we reported the isolation and characterization of the gene, cyc07, which was specifically expressed in the S phase during the cell cycle in synchronous cell division cultures of the higher plant, Catharanthus roseus. We found that the yeast Saccharomyces cerevisiae contains two closely related genes which show a high degree of similarity (about 64% at the amino acid level) to cyc07 of C. roseus. Site-directed disruption mutations demonstrated that the two yeast genes, homologous to cyc07, constitute an essential gene family for cell proliferation in yeast cells. Furthermore, the rate of cell proliferation varied with the gene copy number.

【 授权许可】

Unknown