Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation

FEBS Letters

Barker, Hazel M.²  Dombrádi, Viktor²  Axton, J.Myles¹  Cohen, Patricia T.W.² 

[1] Cancer Research Campaign Cell Cycle Genetics Group, Department of Biochemistry, The University, Dundee DD1 4HN, Scotland, UK;Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, The University, Dundee, DD1 4HN, Scotland UK

关键词: Protein phosphatase;    Drosophila;    Gene sequence;    Mitosis;    Position effect variegation;   

DOI  :  10.1016/0014-5793(90)81434-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Drosophila gene encoding a protein phosphatase 1(PPI) has been sequenced, and lethal mutations in this locus (87B) analysed. Two mutants (ck19 ^e211 and ck19 ^hs46), which disrupt mitosis, lack the 87B isoenzyme and express only ∼20% of wild type PPI activity. The Promoter region of the gene is deleted in the ck19 ^e11 mutant. A third mutant (ck19 ^e078), which shows suppression of position effect variegation, but has little effect on mitosis, possesses ∼35% of wild type PPI activity. The results indicate that the PPI 87B isoenzyme is involved in regulation of chromosome condensation at interphase as well as mitosis.

【 授权许可】

Unknown