Polymerase chain reactions using Saccharomyces, Drosophila and human DNA predict a large family of protein serine/threonine phosphatases

FEBS Letters

Chen, Yu Hua¹  Chen, Mao Xiang¹  Cohen, Patricia T.W.¹ 

[1] Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, The University, Dundee, DD1 4HN, Scotland, UK

关键词: Protein phosphatase;    Gene family;    Polymerase chain reaction;    Saccharomyces;    Drosophila;    human;   

DOI  :  10.1016/0014-5793(92)80836-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the polymerase chain reaction (PCR) to examine the protein serine/threonine phosphatase (PP) family which includes PP1, PP2A and PP2B, we have identified two, seven, and four novel protein phosphate genes in Saccharomyces cerevisiae, Drosophila melanogaster and Homo sapiens, respectively. Consequently, the genes in the PP1/PP2A/PP2B family now number 11, 15 and 12 in these species respectively, and the data predicts still more unidentified phosphatases in higher eukaryotes. The PCR analyses also point to the presence in Drosophila and mammals of three or more different genes encoding PP2B, the enzyme recently identified as the target of certain immunosuppressant drugs.

【 授权许可】

Unknown