Isolation of cDNAs encoding a novel member of the neurotransmitter transporter gene family

FEBS Letters

Mayser, Wolfgang¹  Betz, Heinrich¹  Schloss, Patrick¹ 

[1] Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Deutschordenstr. 46, 6000 Frankfurt-71, Germany

关键词: Neurotransmitter;    Transporter;    Polymerase chain reaction;    Gene family;    Spinal cord;   

DOI  :  10.1016/0014-5793(91)81418-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Degenerate oligonucleotides deduced from two regions of the rat γ-aminobutyric acid (GABA) transporter were used to amplify related sequences from rat spinal cord by polymerase chain reaction (PCR). The resulting product then allowed isolation of two overlapping cDNA clones with an open reading frame encoding a hydrophobic polypeptide of 630 amino acids. This protein, termed NTT, exhibits 38% sequence homology to the GABA and 48% to the noradrenaline transporters and contains 12 putative transmembrane regions. PCR amplification revealed low-level expression of NTT transcripts in spinal cord, but not in brain and cerebellum.

【 授权许可】

Unknown