Primary structure and functional expression of a choline transporter expressed in the rat nervous system

FEBS Letters

Mayser, Wolfgang¹  Betz, Heinrich¹  Schloss, Patrick¹ 

[1] Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, D-6000 Frankfurt/M. 71, Germany

关键词: Choline uptake;    Neurotransmitter;    Transporter;    Xenopus oocyte;    Nervous system;    Rat;   

DOI  :  10.1016/0014-5793(92)80649-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthesis of the neurotransmitter acetylcholine in cholinergic nerve terminals is regulated by a sodium-driven high-affinity choline uptake system in the plasma membrane. We have isolated cDNAs from rat spinal cord and brainstem which encode a choline transporter (CHOT1). The predicted protein shares considerable amino acid identity and several structural features including twelve putative transmembrane regions with other neurotransmitter transporters. Expression of in vitro transcribed CHOT1 RNA in Xenopus oocytes generated Na⁺-dependent choline uptake, which was not seen in control oocytes. Amplification by polymerase chain reaction (PCR) revealed significant amounts of CHOT1 mRNA in brain, cerebellum, spinal cord and, to a lesser extent, heart, but only very low expression in lung, kidney and muscle.

【 授权许可】

Unknown