Novel putative protein tyrosine phosphatases identified by the polymerase chain reaction

FEBS Letters

Nishi, Masahiro¹  Steiner, Donald F.¹  Ohagi, Shinya¹ 

[1] The Department of Biochemistry and Molecular Biology and The Howard Hughes Medical Institute, The University of Chicago, 5841 S. Maryland Avenue, Chicago, IL 60637, USA

关键词: Protein tyrosine phosphatase (human liver);    Gene family;    Polymerase chain reaction;    Tyrosine phosphorylation;    Growth regulatory gene;   

DOI  :  10.1016/0014-5793(90)80400-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein tyrosine phosphatases (PTPases) are a family of enzymes that specifically dephosphorylate phosphotyrosyl residues in selected protein substrates. To more fully understand the regulatory role of protein tyrosine phosphorylation and dephosphorylation in cellular signal transduction, characterization of PTPases is essential. Using the polymerase chain reaction and degenerate oligonucleotide primers corresponding to conserved amino acid sequences within the catalytic domain of PTPases, we have identified 11 PTPase-related human liver cDNA sequences. Five of these have not been described previously. These results indicate that, like protein tyrosine kinases. PTPases may also comprise a gene family with a large number of members.

【 授权许可】

Unknown