Protein phosphorylation is involved in the regulation of chromatin condensation during interphase

FEBS Letters

Dombrádi, Viktor¹  Cohen, Paricia T.W.¹ 

[1] Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, The University, Dundee DD1 4HN, Scotland, UK

关键词: Protein phosphatase;    Chromatin;    Position effect variegation;    Mitosis;    Drosophila;    PP;    protein phosphatase;    PEV;    position effect variegation;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(92)81402-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Loci affecting the condensation state of interphase chromatin have been previously identified from analysis of suppression and enhancement of position effect variegation (PEV) in Drosophila. Here we show that Su-var(3)6 and an allelic mutant, e078, which both show suppression of PEV in the heterozygous state, have point mutations (Gly²²⁰→Ser and Gly²²⁰→Asp, respectively) in a protein phosphatase 1 catalytic subunit located at 87B (PP1 87B). The mutated glycine is conserved in all known protein serine/threonine phosphatases in the same gene family, and its substitution decreases PP1 activity. We conclude that protein dephosphorylation by PP1 87B regulates the condensation state of chromatin during interphase.

【 授权许可】

Unknown