Precise mapping and molecular characterization of the MFT1 gene involved in import of a fusion protein into mitochondria in Saccharomyces cerevisiae

FEBS Letters

Yasui, Akira¹  Komamine, Atsushi²  Ito, Masaki² 

[1] Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai 980, Japan;Biological Institute, Faculty of Science, Tohoku University, Sendai 980, Japan

关键词: Mitochondrial protein import;    MFTI;    cyc07;    Gene sequence;    Saccharomyces cerevisiae;    ORF;    open reading frame;    ts;    temperature sensitive;    YEPD;    yeast extract-peptone-dextrose;   

DOI  :  10.1016/0014-5793(93)80076-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Garrett et al. [Mol. Gen. Genet. 225 (1991) 483-491] recently reported that an Atp2-lacZ fusion protein was transported into mitochondria in yeast, thus identifying the MFT1 (mitochondrial fusion targeting) gene as a genomic fragment which complements a mutation (mftI) that failed in targeting a fusion protein into mitochondria [4]. They mapped this gene to the ORF, which we have independently identified as a gene homologous to the cyc07 gene, which is expressed specifically in the S phase during the plant cell cycle. We have mapped the MFT1 gene precisely and found that this gene should correspond to the neighboring ORF, rather than the ORF they identified.

【 授权许可】

Unknown