Uncoupling effect of fatty acids on heart muscle mitochondria and submitochondrial particles

FEBS Letters

Arrigoni-Martelli, E.³  Starkov, A.A.¹  Bobyleva, V.A.²  Dedukhova, V.I.¹  Skulachev, V.P.¹  Mokhova, E.N.¹ 

[1]Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, U.S.S.R.

[2]Institute of General Pathology, Modena, Italy

[3]Sigma-Tau, Pomesia, Roma, Italy

关键词: Uncoupling;    Fatty acid;    ATP/ADP-antiporter;    Heart mitochondria;    Submitochondrial particle;    Δψ;    transmembrane electric potential difference;    Atr;    atractylate;    BSA;    bovine serum albumin;    CAtr;    carboxyatractylate;    DNP;    2;    4-dinitrophenol;    EDTA;    ethylenediamine-N;    N;    N′N′-tetraacetic acid;    EGTA;    ethylene glycol-bis(β-aminoethyl ether)-N;    N;    N′;    N′-tetraacetic acid;    FCCP;    p-trifluoromethoxycarbonylcyanide phenylhydrazone;    MOPS;    morpholinopropane sulphonate;    PCB−;    phenyldicarbaundecaborane;    SDS;    sodium dodecyl sulfate;    TPP+;    tetraphenyl phosphonium;   

DOI  :  10.1016/0014-5793(91)81382-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of ATP/ADP-antiporter inhibitors on palmitate-induced uncoupling was studied in heart muscle mitochondria and inside-out submitochondrial particles. In both systems palmitate is found to decrease the respiration-generated membrane potential. In mitochondria, this effect is specifically abolished by carboxyatractylate (CAtr) a non-penetrating inhibitor of antiporter. In submitochondrial particles, CAtr does not abolish the palmitate-induced potential decrease. At the same time, bongkrekic acid, a penetrating inhibitor of the antiporter, suppresses the palmitate effect on the potential both in mitochondria and particles. Palmitoyl-CoA which is known to inhibit the antiporter in mitochondria as well as in particles decreases the palmitate uncoupling efficiency in both these systems. These data are in agreement with the hypothesis that the ATP/ADP-antiporter is involved in the action of free fatty acids as natural uncouplers of oxidative phosphorylation.

【 授权许可】

Unknown