Does the function of adenine nucleotide translocase in fatty acid uncoupling depend on the type of mitochondria?

FEBS Letters

Schönfeld, Peter¹ 

[1]Institut für Biochemie der Medizinischen Akademie Magdeburg, Magdeburg, GDR

关键词: Uncoupling;    Fatty acid;    Mitochondria;    Adenine nucleotide translocase;    LCFA;    long-chain fatty acids;    ANT;    adenine nucleotide translocase;    CAT;    carboxyatractyloside;    FCCP;    p-tri-fluoromethoxycarbonylcyanide phenylhydrazone;    RLM;    rat liver mitochondria;    RHM;    rat heart mitochondria;    RKM;    rat kidney mitochondria;   

DOI  :  10.1016/0014-5793(90)80259-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The stimulation of respiration by long-chain fatty adds and FCCP was studied with oligomycin-inhibited mitochondria from rat liver, heart and kidney tissue. By addition of equal amounts of palmitate and oleate, mitochondrial respiration was increased in the order RLM < RKM < RHM. Using the classical protonophore FCCP, this difference could not be observed. Inhibition of oleate-stimulated respiration by carboxyatractyloside decreased in the order RHM > RKM > RLM. As CAT sensitivity of oleate-stimulated respiration and the mitochondrial ANT content were found to be correlated, it is suggested that the weak CAT sensitivity of oleate-stimulated respiration of RLM [(1989) Biochim. Biophys. Acta 977, 266-272] is due to the low content of ANT.

【 授权许可】

Unknown