Inhibitors of the ATP/ADP antiporter suppress stimulation of mitochondrial respiration and H+ permeability by palmitate and anionic detergents

FEBS Letters

Brustovetsky, N.N.¹  Egorova, M.V.¹  Dedukhova, V.I.²  Skulachev, V.P.²  Mokhova, E.N.² 

[1] Institute of Biological Physics, USSR Academy of Science, Pushchino, Moscow Region 142292, USSR;Department of Bioenergetics, A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Fatty acid;    Uncoupling;    ATP/ADP-antiporter;    DNP;    2;    4-p-dinitrophenol;    BSA;    bovine serum albumin;    FCCP;    p-trifluoromethoxycarbonylcyanide phenylhydrazone;    Mops;    morpholinopropane sulphonate;    SDS;    sodium dodecyl sulfate;    CTAB;    cetyltrimethylammonium bromide;    PLP;    pyridoxal 5-phosphate;    CAtr;    carboxyatractylate;   

DOI  :  10.1016/0014-5793(90)80480-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The action of ATP/ADP-antiporter inhibitors upon the uncoupling effect of palmitate, detergents and ‘classical’ uncouplers has been studied. The uncoupling effect was estimated by stimulation of succinate oxidation and of H⁺ permeability of rat liver mitochondria in the presence of oligomycin. It is shown that carboxyatractylate (CAtr) and pyridoxal 5-phosphate (PLP) suppress the uncoupling induced by palmitate and the anionic detergents SDS and cholate, but do not affect that induced by the cationic detergents CTAB, by the non-ionic detergent Triton X-100, as well as by the ‘classical’ uncouplers FCCP and DNP. The results are discussed in terms of a concept assuming that the ATP/ADP-antiporter facilitates the electrophoretic export of hydrophobic anions from mitochondria.

【 授权许可】

Unknown