Carboxyatractylate‐sensitive uncoupling in liver mitochondria from ground squirrels during hibernation and arousal

FEBS Letters

Brustovetsky, N.N.²  Amerkanov, Z.G.²  Yegorova, M.E.²  Skulachev, V.P.¹  Mokhova, E.N.¹ 

[1]A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

[2]Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR

关键词: Hibernation;    Uncoupling;    Fatty acid;    ATP/ADP-antiporter;    BSA;    bovine serum albumin;    CAtr;    carboxyatractylate;    DNP;    2;    4-p-dinitrophenol;    FFA;    free fatty acid;    TPP+;    tetraphenyl phosphonium;    Hepes;    N-(2-hydroxyethyl)piperazine-N' -(2-ethanesulfonic acid);   

DOI  :  10.1016/0014-5793(90)80481-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Energy coupling parameters of liver mitochondria from hibernating and arousing ground squirrels have been studied. In the oligomycin-treated mitochondria, carboxyatractylate, an inhibitor of the ATP/ADP-antiporter, is shown to decrease the respiration rate, to increase the membrane potential and to lower the rate of the membrane-potential discharge after the addition of cyanide to liver mitochondria from hibernating and arousing animals. BSA effectively substitutes for carboxyactactylate so that carboxyactactylate, added after BSA, has no effect. In mitochondria from hibernating animals, the maximal respiration rate in the presence of DNP and the rate of the membrane potential discharge in its absence are much lower than in those from arousing animals. It has been concluded that upon arousal of the animals from hibernation, the uncouling of oxidative phosphorylation, mediated by free fatty acids and ATP/ADP-antiporter, parallels the respiratory chain activation.

【 授权许可】

Unknown