Regulation of ATP hydrolysis in liver mitochondria from ground squirrel

FEBS Letters

Chernyak, B.V.¹  Vinogradova, S.O.¹  Bronnikov, G.E.¹ 

[1] Institute of Biological Physics, USSR Academy of Sciences, Pushchino, USSR

关键词: ATPase inhibitor protein;    Uncoupling;    Calcium ion;    Hibernation;    Liver mitochondria;    Ground squirrel;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    TTFB;    4;    5;    6;    7-tetrachloro-2-trifluoromethyl benzimidazole;   

DOI  :  10.1016/0014-5793(90)81512-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The uncoupler-induced inactivation of H⁺-ATPase in liver mitochondria from ground squirrel has been studied. The dependence of this process on ΔgmH⁺, pH and ATP indicates that it is caused by the protein inhibitor. This conclusion is also supported by the protective effect of Zn²⁺ and Cu²⁺. The inactivation can be induced by Ca²⁺ at low concentrations in the presence of phosphate. It is shown that the protein inhibitor inactivates ATPase almost completely under optimal conditions while its effect in mice or rat liver mitochondria does not exceed 30%. The potential efficiency of the inhibitor's action does not depend on either the season or the state of animals (hibernating or active). At the same time, the sensitivity of this system to Ca²⁺ is significantly lower in active (summer) animals.

【 授权许可】

Unknown