Bacteriochlorophyll c formation via the C5 pathway of 5‐aminolevulinic acid synthesis in Chloroflexus aurantiacus

FEBS Letters

Scott, A.Ian¹  Oh-hama, Tamiko¹  Santander, Patricio J.¹  Stolowich, Neal J.¹ 

[1] Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station, TX 77843-3225, USA

关键词: 5-Aminolevulinic acid synthesis;    C5 pathway;    Bacteriochlorophyll c;    13C NMR;    Chloroflexus aurantiacus;    ALA;    5-aminolevulinic acid;    tricine;    N-tris-(hydroxymethyl)-methylglycine;    NMR;    nuclear magnetic resonance;    HPLC;    high pressure liquid chromatography;   

DOI  :  10.1016/0014-5793(91)80386-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Biosynthesis of 5-aminolevulinic acid (ALA) in Chloroflexus aurantiacus, a thermophilic bacterium forming bacterioclorophyll c, is shown to proceed via the C₅ pathway by demonstrating (1) the specific labeling of its chlorin ring with [1-¹³C] glutamate and (2) the enzyme activity to produce ALA from glutamate in a cell-free extract. From the phylogenetic distribution it is suggested that ALA synthase distributed in some aerobic eubacteria could be monophyletic in origin.

【 授权许可】

Unknown