Neurokinin‐immunoreactivity in human neuroblastomas

FEBS Letters

Gaedicke, Gerhard¹  McGregor, Gerard P.²  Voigt, Karlheinz² 

[1] University Childrens Hospital, Dept Paediatrics II, Prittwitzstr, 43, D-7900 Ulm, Germany;Institute of Physiology, Deutschhausstr. 2, D-3550 Marburg, Germany

关键词: Neuroblastoma;    Tachykinin;    Neurokinin B;    Human;    PNS;    peripheral nervous system;    CNS;    central nervous system;    PPT;    preprotachykinin;    NKA-IR;    neurokinin A-immunoreactivity;    SP-IR;    substance P-IR;    rpHPLC;    reverse-phase high-pressure liquid chromatography;    K a;    affinity constant;    ID50 concentration giving 50% inhibition;   

DOI  :  10.1016/0014-5793(90)80814-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Factors regulating differentiation of the peripheral nervous system (PNS) have been widely studied in neuroblastomas which are tumors of the PNS. Five neuroblastomas were investigated for their content of tachykinin neuropeptides, which arise from two distinct genes which appear differentially expressed in the PNS. Radioimmunoassay and column chromatography revealed large amounts of neurokinin B in three of these tumors and the absence of substance P, neurokinin A, neuropeptide K and neuropeptide γ from all five tumors. This suggests that neuroblastomas can selectively express the preprotachykinin (PPT) II gene and that they may be valuable for investigating the factors involved in the regulation of these two structurally-related neuropeptide genes.

【 授权许可】

Unknown