80% of muscarinic receptors expressed by the NB‐OK 1 human neuroblastoma cell line show high affinity for pirenzepine and are comparable to rat hippocampus M1 receptors

FEBS Letters

Christophe, Jean¹  Tastenoy, Michèle¹  Waelbroeck, Magali¹  Camus, Jean¹ 

[1] Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Bvd de Waterloo 115, B-1000 Brussels, Belgium

关键词: Muscarinic receptor;    Receptor binding assay;    Receptor subtype;    Neuroblastoma;    (NB-OK cell;    Human;    Rat pancreas;    Rat hippocampus);   

DOI  :  10.1016/0014-5793(88)81441-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The NB-OK 1 human neuroblastoma cell line expressed muscarinic cholinergic receptors that could be labeled with N-[³H]methylscopolamine (a nonselective antagonist). 80% of these receptors showed high affinity for pirenzepine, i.e. belonged to the M1 subtype found in neuronal tissues. Their binding properties were identical to those of rat hippocampus M1 receptors, and differed from those of rat pancreas and heart muscarinic receptors. The remaining (20%) muscarinic receptors showed low affinity for pirenzepine and AF-DX 116, being therefore of an M2β (or B) subtype, and were similar to rat pancreatic receptors.

【 授权许可】

Unknown