Somatostatinergic phenotype markers in the human neuroblastoma cell‐line LA‐N‐2

FEBS Letters

Folkesson, Ronnie¹  Påhlman, Sven²  Nilsson, Lars¹  Bergström, Lena³  Winblad, Bengt¹  Meyersson, Gabrielle² 

[1] Department of Clinical Neuroscience and Family Medicine, Division of Geriatric Medicine, Alzheimer's Disease Research Center, Karolinska Institute, Huddinge University Hospital B84, S-141 86 Huddinge, Sweden;Department of Pathology, Uppsala University Hospital, S-751 85 Uppsala, Sweden;Department of Pharmaceutical Biosciences, Division of Pharmacology, Uppsala University, Biomedical Centre, Box 591, S-751 24 Uppsala, Sweden

关键词: Somatostatin;    Neuroblastoma;    Human;    LA-N-2;    mRNA;    Receptor;   

DOI  :  10.1016/0014-5793(95)00958-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have characterized somatostatinergic phenotype markers of the human neuroblastoma, LA-N-2. A single mRNA-transcript (∼850bp) and two cellular somatostatin immunoreactivity forms, a high molecular weight form (M _r 15,000) and a fragment corresponding to somatostatin-28 was found, while the somatostatin-14 peptide was absent. Saturation binding experiments demonstrated a single class of high-affinity somatostatin receptors with K _d and B _max of 0.27 ± 0.03 nM and 45 ± 1 fmol/mg protein. Partial G-protein uncoupling (30%) was demonstrated, using GTPγS, with an affinity of 9.7 nM. The LA-N-2 cell line, previously shown to be cholinergic, may serve as a simplified system to elucidate heterologous neurotransmittor interactions. Such studies are of interest since dysfunctions of the cholinergic basal forebrain neurons and somatostatin immunoreactive interneurons have been consistently observed in Alzheimer's disease.

【 授权许可】

Unknown