Fructose 2,6‐bisphosphate and glycolytic flux in skeletal muscle of swimming frog

FEBS Letters

Wegener, Gerhard¹  Thuy, Michael¹  Krause, Ulrike¹ 

[1] Institut für Zoologie der Johannes Gutenberg-Universität, Saarstrasse 21, D-6500 Mainz, FRG

关键词: Skeletal muscle;    Glycolysis;    Fructose 2;    6-bisphosphate;    6-Phosphofructokinase;    Exercise;    Rana temporaria;    Fl;    6P2;    fructose 1;    6-bisphosphate;    F2;    6P2;    fructose 2;    6-bisphosphate;    G1;    6P2;    glucose 1;    6-bisphosphate;    PFK;    6-phosphofructokinase (EC 2.7.1.11);   

DOI  :  10.1016/0014-5793(90)80939-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glycolytic flux in skeletal muscle is controlled by 6-phosphofructokinase but how this is achieved is controversial. Brief exercise (swimming) in frogs caused a dramatic increase in the phosphofructokinase activator, fructose 2,6-bisphosphate, in working muscle. The kinetics of phosphofructokinase suggest that in resting muscle, the enzyme is inhibited by ATP plus citrate and that the increase in fructose 2,6-bisphosphate is part of the mechanism to activate phosphofructokinase when exercise begins. When exercise was sustained, fructose 2,6-bisphosphate in muscle was decreased as was the rate of lactate accumulation. Glycolytic flux and the content of fructose 2,6-bisphosphate appear to be closely correlated in working frog muscle in vivo.

【 授权许可】

Unknown