A product‐regulated fructose 2,6‐bisphosphatase occurs in green leaves

FEBS Letters

Cséke, Csaba¹  Buchanan, Bob B.¹  Balogh, Árpád¹  Stitt, Mark¹ 

[1] Division of Molecular Plant Biology, Hilgard Hall, University of California, Berkeley, CA 94720, USA

关键词: Fructose 2;    6-bisphosphate;    Fructose 2;    6-bisphosphatase;    Sucrose synthesis;    Gluconeogenesis;    Glycolysis;    Enzyme regulation;    Fru-2;    6-P2;    fructose 2;    6-bisphosphate;    Fru-2;    6-P2ase;    fructose 2;    6-bisphosphatase;    Fru-6-P;    2K;    fructose 6 phosphate;    2-kinase;    PFP;    pyrophosphate-linked phosphofructokinase (EC 2.7.190);    PGA;    3-phosphoglycerate;    Fru-1;    6-P2;    fructose 1;    6-bisphosphate;    Glu-1;    6-P2;    glucose 1;    6-diphosphate;   

DOI  :  10.1016/0014-5793(83)81057-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An enzyme catalyzing the hydrolytic conversion of fructose 2,6-bisphosphate (Fru-2,6-P₂) to fructose 6-phosphate (Fru-6-P) and P_i has been identified and purified from plants, specifically the cytosolic fraction of spinach leaf parenchyma cells. Partially purified preparations of the enzyme, designated fructose 2,6-bisphosphatase (Fru-2,6-P₂ase), were inhibited by products of the reaction (i.e., P_i and Fru-6-P) but showed no response to a protein phosphorylation system known to inhibit the corresponding enzyme in mammalian cells. Fru-2,6-P₂ase co-purified with fructose 6-phosphate,2-kinase, the enzyme catalyzing the synthesis of Fru-2,6-P₂. The observed pattern of regulation of the enzymes functional in the synthesis and breakdown of Fru-2,6-P₂ reinforces the conclusion that chloroplasts play a role in controlling cytosolic carbon processing in leaves.

【 授权许可】

Unknown