Apoptosis induced by growth factor withdrawal in fibroblasts overproducing fructose 2,6‐bisphosphate

FEBS Letters

Hue, Louis¹  Rousseau, Guy G¹  Gueuning, Marie-Agnès¹  Darville, Martine I¹  Durante, Paula¹ 

[1] Hormone and Metabolic Research Unit, Université Catholique de Louvain and Christian de Duve Institute of Cellular Pathology (ICP), 75 Avenue Hippocrate, B-1200 Brussels, Belgium

关键词: 5-Amino-4-imidazolecarboxamide riboside;    5′-AMP-activated protein kinase;    Apoptosis;    Fructose 2;    6-bisphosphate;    6-Phosphofructo-2-kinase/fructose 2;    6-bisphosphatase;    Rat-1 fibroblast;    AICAR;    5-amino-4-imidazolecarboxamide riboside;    AMPK;    5′-AMP-activated protein kinase;    BrdU;    bromodeoxyuridine;    CMV;    cytomegalovirus;    DMEM;    Dulbecco's modified Eagle medium;    FCS;    fetal calf serum;    F-2;    6-P2;    fructose 2;    6-bisphosphate;    FBPase-2;    fructose 2;    6-bisphosphatase;    PBS;    phosphate buffered saline;    PEG;    polyethylene glycol;    PFK-2;    6-phosphofructo-2-kinase;    PKA;    protein kinase A;    PP-2A;    phosphoprotein phosphatase-2A;    TUNEL;    terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling;   

DOI  :  10.1016/S0014-5793(99)00387-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fructose 2,6-bisphosphate is a potent endogenous stimulator of glycolysis. A high aerobic glycolytic rate often correlates with increased cell proliferation. To investigate this relationship, we have produced clonal cell lines of Rat-1 fibroblasts that stably express transgenes coding for 6-phosphofructo-2-kinase, which catalyzes the synthesis of fructose 2,6-bisphosphate, or for fructose 2,6-bisphosphatase, which catalyzes its degradation. While serum deprivation in culture reduced the growth rate of control cells, it caused apoptosis in cells overproducing fructose 2,6-bisphosphate. Apoptosis was inhibited by 5-amino-4-imidazolecarboxamide riboside, suggesting that 5′-AMP-activated protein kinase interferes with this phenomenon.

【 授权许可】

Unknown