Cloning and expression of novel isoforms of 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase from bovine heart

FEBS Letters

Rousseau, Guy G.¹  Vidal, Hubert¹  Hue, Louis¹  Crepin, Karine M.¹  Rider, Mark H.¹ 

[1] Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and Louvain University Medical School, B-1200 Brussels, Belgium

关键词: Fructose-2;    6-bisphosphatase;    Fructose 2;    6-bisphosphate;    Glycolysis;    6-phosphofructo-2-kinase;    Splicing;    Bovine heart;    FBPase-2;    fructose-2;    6-bisphosphatase;    ORF;    open reading frame;    PCR;    polymerase chain reaction;    PFK-2;    6-phosphofructo-2-kinase;    PKA;    cyclic AMP-dependent protein kinase;    PKC: protein kinase C;   

DOI  :  10.1016/0014-5793(93)80898-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Distinct 6-phosphofructo-2-kinase (PFK-2)/fructose 2,6-bisphosphatase (FBPase-2) cDNAs were cloned from bovine heart, showing that PFK-2/FBPase-2 gene B, which contains 16 exons, codes for at least five mRNAs. Three of them (B1, B2, B4) could encode the 58,000-M _r isozyme. In B2 mRNA, exon 15 encodes four more residues than in Bl. In B4 mRNA, exon 15 encodes six more residues than in B1, butexon 16 (20 residues) is missing. B3 mRNA corresponds to the 54,000-M _r isozyme. It lacks exon 15 and also differs from the other mRNAs in the 5' noncoding region. B5 mRNA encodes a truncated form. When expressed in E. coli, the recombinant isoforms corresponding to all these mRNAs except B5 exhibited PFK-2 activity.

【 授权许可】

Unknown