Molecular cloning and tissue‐specific expression of mouse kidney 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase

FEBS Letters

Batra, Ritu Sonia¹  Craig, Ian W.¹  Brown, Ruth¹  Brown, Garry K.¹ 

[1] Genetics Laboratory, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU UK

关键词: Alternative splicing;    Fructose-2;    6-bisphosphatase;    Fructose 2;    6-bisphosphate;    Glycolysis;    6Phosphofructo-2-kinase;    Mouse kidney;    bp;    base pairs;    FBPase-2;    fructose-2;    6-bisphosphatase;    Fru-2;    6-P2;    fructuse 2;    6-bisphosphate;    kb;    kilobases;    PFK-2;    6-phosphofructo-2-kinase;   

DOI  :  10.1016/0014-5793(96)00878-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 1932 bp cDNA clone encoding a novel isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) was isolated from a mouse kidney cDNA library. The sequence encodes 519 amino acids and, based on homology to rat heart genomic sequence, appears to be the product of alternative splicing from PFK-2/FBPase-2 gene B with an extended version of exon 15. Northern blot analysis indicated that this clone corresponds to an 8 kb mRNA expressed in multiple tissues, with the strongest signal in kidney, and detects several additional transcripts which may be alternatively spliced from gene B.

【 授权许可】

Unknown