Cells overexpressing fructose‐2,6‐bisphosphatase showed enhanced pentose phosphate pathway flux and resistance to oxidative stress

FEBS Letters

Perez, Xavier³  Boada, Jordi³  Cascante, Marta²  Bartrons, Ramon³  Bermúdez, Jordi³  Roig, Teresa³  Gamez, Antonio¹ 

[1] Departament de Enginyeria Quı́mica, EUETIB, Universitat Politècnica de Catalunya, Catalunya, Spain;Departament de Bioquı́mica i Biologia Molecular, Divisió de Ciències Experimentals, Universitat de Barcelona, Barcelona, Spain;Departament de Ciències Fisiològiques II, Divisió de Ciències de la Salut, Universitat de Barcelona, Feixa Llarga s/n, E-08907 L'Hospitalet de Llobregat, Barcelona, Spain

关键词: Glycolysis;    6-Phosphofructo-2-kinase;    Fructose-2;    6-bisphosphatase;    Fructose-2;    6-bisphosphate;    Pentose phosphate pathway;    Microcalorimetry;    PRPP;    5-phosphoribosyl-1-pyrophosphate;    PFK-2/FBPase-2;    6-phosphofructo-2-kinase/fructose-2;    6-bisphosphatase;    F6P;    fructose-6-phosphate;    Fru-2;    6-P2;    fructose-2;    6-bisphosphate;    Gluc-6P;    glucose-6-phosphate;    G6PDH;    glucose-6-phosphate dehydrogenase;    G3P;    glyceraldehyde-3-phosphate;    PPP;    pentose phosphate pathway;    pentoses-5-P;    pentoses-5-phosphate;    TK;    transketolase;   

DOI  :  10.1016/S0014-5793(00)01950-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Changes in the content of fructose-2,6-bisphosphate, a modulator of glycolytic flux, also affect other metabolic fluxes such as the non-oxidative pentose phosphate pathway. Since this is the main source of precursors for biosynthesis in proliferating cells, PFK-2/FBPase-2 has been proposed as a potential target for neoplastic treatments. Here we provide evidence that cells with a low content of fructose-2,6-bisphosphate have a lower energy status than controls, but they are also less sensitive to oxidative stress. This feature is related to the activation of the oxidative branch of the pentose phosphate pathway and the increased production of NADPH.

【 授权许可】

Unknown