Complete nucleotide sequence coding for rat liver 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase derived from a cDNA clone

FEBS Letters

Rider, M.H.¹  Van Damme, J.²  Crepin, K.M.¹  Darville, M.I.¹  Hue, L.¹  Marchand, M.J.¹  Vandekerckhove, J.²  Rousseau, G.G.¹  Octave, J.N.³ 

[1] Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Rijksuniversiteit, B-9000 Gent, Belgium;Laboratory of Genetics, Rijksuniversiteit, B-9000 Gent, Belgium;Unité de Biologie et de Pharmacologie Cellulaires, Louvain University Medical School, B-1200 Brussel Belgium

关键词: cDNA;    6-Phosphofructo-2-kinase;    Fructose-2;    6-bisphosphatase;    (Rat liver);   

DOI  :  10.1016/0014-5793(87)80476-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA clones for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were isolated from rat liver expression libraries in λgt11 by antibody, oligonucleotide, and cDNA screening. One 1860 bp long clone contained a full-length nucleotide sequence coding for the 470 amino acids of each of the two identical subunits of the bifunctional enzyme. This clone also contained untranslated sequences, one 173 bp long upstream from the ATG start codon and one 271 bp long downstream from the TGA stop codon. The clone was terminated by a poly(A) tail of 29 nucleotides.

【 授权许可】

Unknown