Starvation or diabetes decreases the content but not the mRNA of 6‐phosphofructo‐2‐kinase in rat liver

FEBS Letters

Darville, Martine I.¹  Rousseau, Guy G.¹  Hue, Louis¹  Crepin, Karine M.¹ 

[1] Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and Louvain University Medical School, B-1200 Brussels, Belgium

关键词: mRNA;    cDNA;    6-Phosphofructo-2-kinase;    Fructose 2;    6-bisphosphate;    Diabetes;    (Rat liver);    fructose 2;    6-bisphosphate;    fructose 2;    6-bisphosphate;    PFK-2;    6-phosphofructo-2-kinase;    FBPase-2;    fructose-2;    6-bisphosphatase;    PEG;    polyethylene glycol;   

DOI  :  10.1016/0014-5793(88)80884-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rat liver, the activity of 6-phosphofructo-2-kinase (PFK-2) decreases upon starvation and in diabetes. Cyclic AMP-dependent phosphorylation of the enzyme is not sufficient to account for this decrease. PFK-2 content was therefore measured by immunotitration and relative PFK-2 mRNA levels were determined by hybridization with cDNA probes. The data are compatible with a posttranscriptional mechanism of regulation that involves decreased translational efficiency of PFK-2 mRNA and(or) increased turnover of the PFK-2 protein.

【 授权许可】

Unknown