Identification of two forms of 6‐phosphofructo‐2‐kinase in yeast

FEBS Letters

Gancedo, Carlos¹  Aragón, Juan J.¹  Gómez, María-Esther¹ 

[1] Depto de Bioquímica, Facultad de Medicina UAM, E-28029 Madrid, Spain

关键词: 6-Phosphofructo-2-kinase;    Fructose 2;    6-bisphosphate;    (Yeast);   

DOI  :  10.1016/0014-5793(87)80563-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two forms of 6-phosphofructo-2-kinase have been identified in Saccharomyces cerevisiae by their different chromatographic behaviour on CM-Sephadex C-50. One of them was not adsorbed and represented ∼ 30% of the eluted activity. The other one emerged at about 120 mM KCl. A molecular mass of 120 kDa was found for both of them. No differences in kinetic behaviour in susceptibility to activation by cAMP-dependent protein kinase were found between the two forms.

【 授权许可】

Unknown