Regulation of fructose 2,6‐bisphosphate levels in cold‐acclimated brown adipose tissue of rat

FEBS Letters

Sobrino, F.¹  Pintado, E.¹  Gualberto, A.¹ 

[1] Departamento de Bioquímica, Facultad de Medicina, Universidad de Sevilla, Av. Sanchez Pizjuan, 4, 41009 Sevilla, Spain

关键词: Fructose 2;    6-bisphosphate;    Thyroid hormone;    6-Phosphofructo-2-kinase;    Pyruvate kinase;    (Brown adipose tissue);    Fru 2;    6-P2;    fructose 2;    6-bisphosphate;    PFK-1;    6-phosphofructo-1-kinase (EC 2.7.1.11);    PFK-2;    6-phosphofructo-2-kinase (EC 2.7.1. 105);    BAT;    brown adipose tissue;   

DOI  :  10.1016/0014-5793(88)80804-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of cold exposure and T₄, administration on fructose 2,6-bisphosphate levels, phosphofructokinase-2 and pyruvate kinase activities were examined in rat brown adipose tissue. Cold adaptation (14 days) gave rise to a 2-fold increase in the amount of fructose 2,6-bisphosphate and phosphofructokinase-2 activity, and increased the pyruvate kinase activity 4-fold. If, in addition, the cold-acclimated rats were treated with T₄, these parameters were again significantly enhanced. The effect on phosphofructokinase-2 was on the V _max, without modification of the K _m (for both fructose 6-phosphate and ATP) of the enzyme. In the hypothyroid state, however, the activity of pyruvate kinase remains unchanged. These data support previous observations on stimulation of glycolytic flux during cold adaptation in brown adipose tissue, and a permissive role of thyroid hormones in the process.

【 授权许可】

Unknown