Fructose 2,6‐bisphosphate in isolated foetal hepatocytes

FEBS Letters

Boscá, Lisardo¹  Martín-Sanz, Paloma¹  Cascales, María¹ 

[1] Instituto de Bioquímica (Centro Mixto del CSIC), Facultad de Farmacia. Universidad Complutense, Plaza de Ramón y Cayal s/n, 28040 Madrid, Spain

关键词: 6-Phosphofructo-2-kinase;    Fructose 2;    6-bisphosphate;    Glucagon;    (Fetal hepatocyte);    Fru 2;    6-P2;    fructose 2;    6-bisphosphate;    PFK-2;    6-phosphofructo-2-kinase (EC 2.7.1.-);    PFK-1;    6-phosphofructo-1-kinase (EC 2.7.1.11);    PK;    pyruvate kinase (ATP:pyruvate O 2-phosphotransferase;    EC 2.7.1.40);   

DOI  :  10.1016/0014-5793(87)81127-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fru 2,6-P₂ was present in isolated foetal hepatocytes at a concentration of 1.6 nmol per g cells. When foetal hepatocytes were exposed to glucagon no changes were observed either in the concentration of Fru 2,6-P₂ and lactate release or in the activities of 6-phosphofructo-2-kinase and pyruvate kinase. Incubation of purified 6-phosphofructo-2-kinase with the catalytic subunit of protein kinase did not change the enzyme activity. The inhibition by sn-glycerol 3-phosphate was much lower for the foetal than for adult enzyme. These results suggest that an isoenzyme of 6-phosphofructo-2-kinase in foetal hepatocytes different from that of adult hepatocytes may be present.

【 授权许可】

Unknown