Complete amino acid sequence of fetal bovine serum acetylcholinesterase and its comparison in various regions with other cholinesterases

FEBS Letters

Smyth, K.K.¹  Gentry, M.K.¹  De La Hoz, D.M.¹  Deal, C.D.¹  Rush, R.S.¹  Christner, C.E.¹  Ogert, R.A.¹  Wolfe, A.D.¹  Chapman, T.C.¹  Doctor, B.P.¹ 

[1] Division of Biochemistry, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA

关键词: Acetylcholinesterase;    Amino acid sequence;    Sequence homology;    Serine esterase;   

DOI  :  10.1016/0014-5793(90)81522-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of a mammalian acetylcholinesterase from fetal bovine serum (FBS AChE) is presented. This enzyme has a high degree of sequence identity with other cholinesterases, liver carboxyesterases, esterase-6, lysophospholipase, and thyroglobulin. The locations of 191 amino acids in 10 regions of the FBS enzyme were compared with corresponding sequences of Torpedo, human, and Drosophila AChEs and human serum butyrylcholinesterase (BChE). In one region there is a marked difference in both the number of amino acids and their sequence between mammalian AChE and other AChEs and the human serum BChE. The amino acid sequence of FBS AChE showed overall homologies of 90% with human AChE, 60% with T. californica AChE, 50% with human serum BChE, and 39% with Drosophila AChE in these regions.

【 授权许可】

Unknown