Ag electrode modified with polyhexamethylene biguanide stabilized silver nanoparticles: a new type of SERS substrates for detection of enzymatically generated thiocholine

3rd International Youth Conference on Interdisciplinary Problems of Nanotechnology, Biomedicine and Nanotoxicology

物理学;医药卫生;生态环境科学

Tepanov, A.A.^1 ; Nechaeva, N.L.^1 ; Prokopkina, T.A.^1 ; Kudrinskiy, A.A.^1 ; Kurochkin, I.N.^1 ; Lisichkin, G.V.^1

Lomonosov Moscow State University, Leninskiye Gory, 1, Moscow

119991, Russia^1

关键词: Acetylcholinesterase;    Butyrylcholinesterase;    Cholinesterase activity;    Polyhexamethylene biguanide;    Sers detections;    Sers spectroscopies;    SERS substrate;    Submicromolar concentrations;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/98/1/012002/pdf
DOI  :  10.1088/1757-899X/98/1/012002

来源: IOP

【 摘 要 】

The detection of thiocholine is one of the most widespread techniques for estimation of the cholinesterase activity - acetylcholinesterase and butyrylcholinesterase. Both cholinesterases can be inhibited by organophosphates and carbamates and accordingly can be considered for estimation of these pollutants in the environment. In the current work, SERS spectroscopy was applied for the thiocholine detection. The Ag electrodes modified with silver nanoparticles stabilized by polyhexamethylene biguanide were for the first time suggested as SERS-substrates for that purpose. Such electrodes can be applicable for SERS detection of submicromolar concentrations of thiocholine.