The interaction of amiloride with acetylcholinesterase and butyrylcholinesterase

FEBS Letters

Zemach, Lucie¹  Shalitin, Yechiel¹  Segal, Dina¹ 

[1] Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel

关键词: Acetylcholinesterase;    Butyrylcholinesterase;    Amiloride;    Inhibition;    Rate enhancement;    AChE;    acetylcholinesterase;    BChE;    butyrylcholinesterase;    pNP;    p-nitrophenyl;    oNP;    o-nitrophenyl;    pNPB;    p-nitrophenyl butyrate;    MSF;    methanesulfonyl fluoride;    2-PAM;    pyridine-2-aldoxime methiodide;   

DOI  :  10.1016/0014-5793(90)80730-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The diuretic drug amiloride was found to be a powerful inhibitor of the reaction of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with their specific choline ester substrates. The inhibition constant is in the micromolar range. On the other hand, when added to a mixture of cholinesterase (AChE and BChE) and neutral substrates, amiloride, in some cases, enhanced the reaction rate. The rate of the reaction of butyrylcholinesterase with p-nitrophenyl butyrate was increased up to 12 fold by amiloride.

【 授权许可】

Unknown