Optimization of sol-gel medium for entrapment of acetylcholinesterase enzyme in biosensor for pesticide detection

International Conference on Green Agro-industry and Bioeconomy 2017

农业科学;生物科学;经济学

Wijayanti, S.D.^1 ; Rahayu, F.S.^1 ; Widyaningsih, T.D.^1

Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, Indonesia^1

关键词: Acetylcholinesterase;    Acetylcholinesterase enzymes;    Biosensor applications;    Conventional methods;    Immobilized enzyme activity;    Laboratory technicians;    Pesticide detection;    Response surface methodology;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/131/1/012045/pdf
DOI  :  10.1088/1755-1315/131/1/012045

来源: IOP

【 摘 要 】

Pesticides are chemical substances used to kill and control pests or diseases that can damage crops. The use of pesticides should be done precisely because the accumulation of chemicals contained in pesticides can cause various health effects. Therefore, detection of pesticide residues on plants is important to reduce the risk of poisoning due to pesticide residues. Some of the conventional methods that have been done to detect pesticide residues have weaknesses among expensive tools, takes a long time, and are generally performed by trained laboratory technicians. Biosensors are analytical devices that can measure the quantitative or semi-quantitative targets of analyte by utilizing a bioreceptor such as enzyme. Several studies have shown that enzyme-based acetylcholinesterase-based biosensors can be used to detect pesticide residues in vegetable samples. The objective of this research was to get a proper silica based sol-gel formulation with molar ratio of H2O:TEOS and NaOH concentration as immobilization medium of acetylcholinesterase enzyme for biosensor application. Response Surface Methodology (RSM) was used in order to determine the interaction between the parameters studied and resulting responses which were amount and activity of acetylcholinesterase enzyme. Based on the research, the best result for immobilized enzyme activity was shown by molar ratio (H2O: TEOS) 1: 8 and 4 mM NaOH treatment.