The Effect of Aptamer Concetration towards Reduced Graphene Oxide-Field Effect Transistor Surface Channel for Biosensor Application

Malaysian Technical Universities Conference on Engineering and Technology 2017

Zainol Abidin, Azrul Syafiq^1 ; Rahim, Ruslinda Abdul^1 ; Huan, Chow Yong^2 ; Mohd Maidin, Nur Nasyifa^1 ; Ahmad, Nurul Atiqah^1 ; Ba Hashwan, Saeed S.^1 ; Mohd Faudzi, Fatin Nabilah^1 ; Hong, Voon Chun^1

Institute of Nano Electronic Engineering, Malaysia^1

School of Microelectronic Engineering, Universiti Malaysia Perlis (UniMAP), Kangar Perlis

01000, Malaysia^2

关键词: Biosensing applications;    Biosensing platforms;    Biosensor applications;    Electrical performance;    Reduced graphene oxides;    Reduced graphene oxides (RGO);    Small molecules;    Source and drains;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/318/1/012045/pdf
DOI  :  10.1088/1757-899X/318/1/012045

来源: IOP

【 摘 要 】

Aptamer are artificially produce bioreceptor that has been developed to bind with various target biomolecules such as ion, cells, protein and small molecules. In this research, an aptamer concentration of 0.5 nM, 1 nM, 5 nM, 10 nM, and 50 nM were immobilized on reduced graphene oxide (rGO) integrated with field effect transistor (FET) respectively to study the effect of aptamer concentration toward rGO surface for stable biosensing platform. The 0.5 nM concentration of aptamer shows the highest current result of 84.3 μA at 1 V applied through the source and drain. After immobilized with aminated aptamer, the conductivity shows significant reduction due to the formation of amide bond on rGO surface between aminated aptamer and carboxyl group on rGO. The electrical performance of FET integrated with rGO shows stable electrical performance suitable to be used in the biosensing application.