Antibody conjugated graphene nanocomposites for pathogen detection

India-Japan Expert Group Meeting on Biomolecular Electronics & Organic Nanotechnology for Environment Preservation

物理学;生物科学;生态环境科学

Sign, Chandan^1,2 ; Sumana, Gajjala^1

Biomedical Instrumentation Section, Materials Physics and Engineering Division, National Physical Laboratory, Council of Scientific and Industrial Research, Dr. K. S. Krishnan Road, New Delhi

110142, India^1

Academy of Scientific and Innovative Research (AcSIR), CSIR-National Physical Laboratory Campus, Dr. K. S. Krishnan Marg, New Delhi

110142, India^2

关键词: Biosensing applications;    Carbodiimide couplings;    Coated glass substrates;    ELectrochemical detection;    Electrophoretic deposition techniques;    Food-borne pathogens;    Graphene nanocomposites;    Salmonella typhimurium;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/704/1/012014/pdf
DOI  :  10.1088/1742-6596/704/1/012014

来源: IOP

【 摘 要 】

Graphene oxide (GO), due to its excellent electrochemical properties and large surface area, known to be highly suitable material for biosensing application. Here, we report in situ synthesis of silver nanopaticles (AgNPs) onto the GO sheets for the electrochemical detection of Salmonella typhimurium (S.typhimurium). The GO-AgNPs composites have been deposited onto the indium tin oxide (ITO) coated glass substrate by the electrophoretic deposition technique. Carbodiimide coupling (EDC-NHS) has been used for the immobilization of antibodies of Salmonella typhimurium (anti-S.typhimurium) for detection of S.typhimurium. The electron microscopy and UV-visible studies reveal successful synthesis GO-AgNPs composites while FT-IR studies suggest the proper immobilization of anti-S.typhi. The cyclic voltammetry (CV) has been utilized for detection using anti-S.typhi/GOAgNPs/ITO based immunoelectrode as a function of S.typhimurium concentration. The fabricated immunosensor shows improved sensitivity of 33.04 μACFU-1mLcm-2in a wide detection range of 101to 106CFUmL-1. This immunosensor may be utilized for the detection of other food borne pathogens like aflatoxin and E.coli also.