Role of butyrylcholinesterase in canine tracheal smooth muscle function

FEBS Letters

Adler, Michael¹  Filbert, Margaret G.¹ 

[1] Neurotoxicology Branch, Pathophysiology Division, US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425, USA

关键词: Pseudocholinesterase;    Acetylcholinesterase;    Tetraisopropylpyrophosphoramide;    BW 284C51;   

DOI  :  10.1016/0014-5793(90)80300-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE) in regulating acetylcholine (ACh) lifetime was investigated by use of selective cholinesterase (ChE) inhibitors. Addition of 1 μM tetraisopropylpyrophosphoramide (iso-OMPA) led to a 98% inhibition of BuChE activity with little or no effect on AChE activity. This inhibition wa accompanied by a 26% increase in the amplitude and a 43% prolongation in the half-relaxation time of contractions elicited by electric field stimulation (EFS). Coapplication of BW 284C51 (a selective AChE inhibitor) and 1 μM iso-OMPA resulted in increases of 2-fold in the amplitude and 10-fold in the half-relaxation time of EFS-induced contractions. These alterations were accompanied by small but sustained baseline contractures that were antagonized completely by incubation with exogenous BuChE (2.5 ). The results suggest that BuChE serves to coregulate the lifetime of ACh in canine tracheal smooth muscle.

【 授权许可】

Unknown